Besra G S, Gurcha S S, Khoo K H, Morris H R, Dell A, Hamid M E, Minnikin D E, Goodfellow M, Brennan P J
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
Zentralbl Bakteriol. 1994 Nov;281(4):415-32. doi: 10.1016/s0934-8840(11)80328-2.
Representative strains of M. senegalense and an unusual strain, labelled M. farcinogenes (M280) were examined by thin-layer chromatography for the presence of characteristic surface glycolipids. In the case of M. farcinogenes M280 and M. senegalense M264, the glycolipids were of the alkali-labile acyltrehalose lipooligosaccharide (LOS) class of antigens, whereas M. senegalense M263 was found to contain the alkali-stable glycopeptidolipids (GPL). Through a combination of 1H-NMR, methylation analysis, FAB/MS, and other analytical techniques, the structures of these glycolipids were deduced. The LOS glycolipids were found to be similar in structure to the characteristic glucosyltrehalose-based glycolipids isolated previously from clinical isolates of M. fortuitum, but distinct from the diacyltrehaloses characteristic of the type strain of M fortuitum. The glycopeptidolipids from M. senegalense M263 were closely similar to those characterized previously from M.
利用薄层色谱法检测了塞内加尔分枝杆菌的代表性菌株以及一株标记为产鼻疽分枝杆菌（M280）的异常菌株，以确定其是否存在特征性表面糖脂。就产鼻疽分枝杆菌M280和塞内加尔分枝杆菌M264而言，其糖脂属于碱不稳定酰基海藻糖脂寡糖（LOS）类抗原，而塞内加尔分枝杆菌M263被发现含有碱稳定糖肽脂（GPL）。通过结合¹H-NMR、甲基化分析、快原子轰击质谱（FAB/MS）及其他分析技术，推断出了这些糖脂的结构。结果发现，LOS糖脂的结构与先前从偶然分枝杆菌临床分离株中分离出的基于葡萄糖基海藻糖的特征性糖脂相似，但与偶然分枝杆菌模式菌株特有的二酰基海藻糖不同。塞内加尔分枝杆菌M263的糖肽脂与先前从[此处原文缺失分枝杆菌名称]中鉴定出的糖肽脂非常相似。
