López Marín L M, Lanéelle M A, Promé D, Daffé M
Département III du LPTF-CNRS, Toulouse, France.
Eur J Biochem. 1993 Aug 1;215(3):859-66. doi: 10.1111/j.1432-1033.1993.tb18103.x.
The structures of the major glycolipid antigens of two animal pathogens Mycobacterium senegalense and Mycobacterium porcinum were elucidated by a combination of fast-atom bombardment mass spectrometry, nuclear magnetic resonance spectroscopy, chemical analyses and radiolabeling experiments. Five glycoconjugates belonging to the class of C-mycoside glycopeptidolipids were characterized in each species. They shared with those recently described in M. peregrinum the same unusual distribution of the disaccharides on the alaninol end of the molecules. Both species showed the presence of the novel sulfated glycopeptidolipid. In addition, some acetylated forms of the glycolipids were also present in the species examined. Identical seroreactivities were observed between the glycolipid antigens extracted from M. senegalense, M. porcinum and M. peregrinum and an antiserum raised against the whole lipid antigens of M. peregrinum. These data reinforce the close taxonomic relationships between the three mycobacterial species and demonstrate the antigenicity of the new variants of mycobacterial glycopeptidolipids.
通过快原子轰击质谱、核磁共振光谱、化学分析和放射性标记实验相结合的方法，阐明了两种动物病原体——塞内加尔分枝杆菌和猪分枝杆菌主要糖脂抗原的结构。在每个物种中鉴定出了五种属于C-霉菌糖苷糖肽脂类的糖缀合物。它们与最近在 Peregrinum 分枝杆菌中描述的那些在分子丙氨醇末端的二糖分布相同。两个物种都显示出新型硫酸化糖肽脂的存在。此外，在所检测的物种中也存在一些糖脂的乙酰化形式。从塞内加尔分枝杆菌、猪分枝杆菌和 Peregrinum 分枝杆菌中提取的糖脂抗原与针对 Peregrinum 分枝杆菌全脂抗原产生的抗血清之间观察到相同的血清反应性。这些数据加强了这三种分枝杆菌物种之间密切的分类学关系，并证明了分枝杆菌糖肽脂新变体的抗原性。
