Gonzalez D H, Bonnard G, Grienenberger J M
Institute de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Curr Genet. 1993 Sep;24(3):248-55. doi: 10.1007/BF00351799.
Sequence analysis of a transcribed region of the wheat mitochondrial (mt) genome revealed two open reading frames (orfs) coding for proteins of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb RNA. The largest orf codes for a hydrophobic protein which bears similarity to a bacterial protein involved in the biogenesis of c-type cytochromes. Its corresponding RNA sequence is fully edited at 34 positions. The second orf encodes a protein homologous to the amino-terminal third of E. coli ribosomal protein S1, corresponding to the ribosome-binding domain of this protein. Its RNA sequence is edited at four positions, one of the edits creating a stop codon. The presence of both proteins in wheat mitochondria was demonstrated using specific antibodies raised against fusion proteins obtained in E. coli from the corresponding cDNAs.
对小麦线粒体（mt）基因组转录区域的序列分析揭示了两个开放阅读框（orf），分别编码由589个和174个氨基酸组成的蛋白质。这两个基因在一个2.6 kb的RNA中共转录。最大的orf编码一种疏水蛋白，该蛋白与参与c型细胞色素生物合成的细菌蛋白相似。其相应的RNA序列在34个位置被完全编辑。第二个orf编码一种与大肠杆菌核糖体蛋白S1氨基末端三分之一同源的蛋白质，对应于该蛋白的核糖体结合结构域。其RNA序列在四个位置被编辑，其中一个编辑产生了一个终止密码子。利用针对从相应cDNA在大肠杆菌中获得的融合蛋白产生的特异性抗体，证实了这两种蛋白在小麦线粒体中的存在。
