Schuster W, Combettes B, Flieger K, Brennicke A
Institut für Genbiologische Forschung, Berlin, Germany.
Mol Gen Genet. 1993 May;239(1-2):49-57. doi: 10.1007/BF00281600.
Analysis of a transcribed region in the mitochondrial genome of Oenothera revealed an open reading frame (ORF) of 577 codons (orf577) that is also conserved in carrot, here encoding a protein of 579 amino acids (orf579). RNA editing alters the mRNA sequence of orf577 in Oenothera with 46 C to U transitions, many of which improve sequence similarity with the homologous Marchantia gene orf509. The deduced polypeptides show significant similarity with the ccl1-encoded protein involved in cytochrome c biogenesis in the photosynthetic bacterium Rhodobacter capsulatus. A highly conserved domain is also found in plastid ORFs, suggesting that these bacterial, chloroplast and mitochondrial genes encode polypeptides with analogous functions in assembly and maturation of cytochromes c.
对月见草线粒体基因组转录区域的分析揭示了一个由577个密码子组成的开放阅读框（orf577），该阅读框在胡萝卜中也保守存在，在此处编码一个由579个氨基酸组成的蛋白质（orf579）。RNA编辑改变了月见草中orf577的mRNA序列，发生了46个C到U的转变，其中许多转变提高了与同源地钱基因orf509的序列相似性。推导的多肽与参与光合细菌红假单胞菌细胞色素c生物合成的ccl1编码蛋白具有显著相似性。在质体开放阅读框中也发现了一个高度保守的结构域，这表明这些细菌、叶绿体和线粒体基因编码的多肽在细胞色素c的组装和成熟中具有类似的功能。
