Puisieux A, Galvin K, Troalen F, Bressac B, Marcais C, Galun E, Ponchel F, Yakicier C, Ji J, Ozturk M
Laboratory of Molecular Oncology, Centre Leon Berard, Lyon, France.
FASEB J. 1993 Nov;7(14):1407-13. doi: 10.1096/fasebj.7.14.8224613.
We analyzed the status of retinoblastoma and p53 genes in 10 human hepatoma cell lines. Polyclonal anti-peptide antibodies generated against peptides homologous to COOH-terminal and leucine-zipper domains of the retinoblastoma protein allowed us to identify two cell lines (Hep 3B and FOCUS) with abnormal expression. The same cell lines have both lacked p53 expression. In contrast to the retinoblastoma gene, the expression of the p53 gene was abnormal in six additional cell lines. Indeed, only the Hep G2 hepatoblastoma cell line (and its derivative Hep G2/2215) appeared to have normal p53 and retinoblastoma gene expression. Our studies indicate that p53 abnormalities are common but retinoblastoma gene aberrations are rare in human hepatoma cell lines.
我们分析了10种人肝癌细胞系中视网膜母细胞瘤基因和p53基因的状态。针对与视网膜母细胞瘤蛋白的COOH末端和亮氨酸拉链结构域同源的肽段产生的多克隆抗肽抗体，使我们能够鉴定出两个表达异常的细胞系（Hep 3B和FOCUS）。相同的细胞系均缺乏p53表达。与视网膜母细胞瘤基因不同，另外六个细胞系中p53基因的表达异常。实际上，只有Hep G2肝母细胞瘤细胞系（及其衍生物Hep G2/2215）似乎具有正常的p53和视网膜母细胞瘤基因表达。我们的研究表明，在人肝癌细胞系中，p53异常很常见，但视网膜母细胞瘤基因畸变很少见。
