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大肠杆菌染色体的glnB区域。

The glnB region of the Escherichia coli chromosome.

作者信息

Liu J, Magasanik B

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

J Bacteriol. 1993 Nov;175(22):7441-9. doi: 10.1128/jb.175.22.7441-7449.1993.

DOI:10.1128/jb.175.22.7441-7449.1993
PMID:8226691
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206889/
Abstract

We present sequences of the glnB gene of Escherichia coli and of two open reading frames (ORFs) located directly upstream of glnB and transcribed in the same direction. The major transcriptional start sites for glnB are located between ORF-2 and glnB, but some transcription of glnB is initiated at the promoter for ORF-1. The putative amino acid sequence of the ORF-2 product has high homology to that of response regulators which by phosphorylation acquire the ability to activate transcription of sigma 54-dependent promoters. The product of ORF-1 showed no similarity to other known proteins. The product of neither ORF-1 nor ORF-2 is necessary for the ability of PII, the product of glnB, to bring about the repression of glutamine synthetase in response to nitrogen excess. On the other hand, the product of hmpA, a gene located on the other side of glnB and transcribed in the opposite direction, appears to play an auxiliary role in this process.

摘要

我们展示了大肠杆菌glnB基因以及位于glnB上游且同向转录的两个开放阅读框（ORF）的序列。glnB的主要转录起始位点位于ORF - 2和glnB之间，但glnB的一些转录起始于ORF - 1的启动子。ORF - 2产物的推测氨基酸序列与应答调节因子的序列具有高度同源性，应答调节因子通过磷酸化获得激活依赖于σ54启动子转录的能力。ORF - 1的产物与其他已知蛋白质没有相似性。对于glnB的产物PII响应氮过量导致谷氨酰胺合成酶的抑制能力而言，ORF - 1和ORF - 2的产物都不是必需的。另一方面，位于glnB另一侧且反向转录的hmpA基因的产物似乎在此过程中起辅助作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ea/206889/c67ecaf1cec5/jbacter00064-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ea/206889/e4ac0e88a3f8/jbacter00064-0331-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ea/206889/c67ecaf1cec5/jbacter00064-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ea/206889/e4ac0e88a3f8/jbacter00064-0331-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ea/206889/c67ecaf1cec5/jbacter00064-0332-a.jpg

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本文引用的文献

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Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor.嘌呤阻遏物对大肠杆菌glnB、prsA和speA的调控
J Bacteriol. 1993 Jun;175(11):3598-606. doi: 10.1128/jb.175.11.3598-3606.1993.
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Characterization of translational initiation sites in E. coli.大肠杆菌中转录起始位点的表征
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Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.含有大肠杆菌glyA基因的杂交质粒的构建与表达。
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P(II) signal transduction proteins, pivotal players in microbial nitrogen control.P(II) 信号转导蛋白，微生物氮控制中的关键参与者。
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The global nitrogen regulator NtcA regulates transcription of the signal transducer PII (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the Cyanobacterium synechococcus sp. strain PCC 7942.全球氮调节因子NtcA调节信号转导蛋白PII（GlnB）的转录，并响应集胞藻PCC 7942菌株中氮和碳的供应情况影响其磷酸化水平。
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The signal transduction protein GlnK is required for NifL-dependent nitrogen control of nif gene expression in Klebsiella pneumoniae.信号转导蛋白GlnK是肺炎克雷伯菌中固氮基因表达的NifL依赖性氮控制所必需的。
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Gene. 1981 Jun-Jul;14(1-2):63-72. doi: 10.1016/0378-1119(81)90148-7.
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Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.一个基因（glnL）的特性，其产物参与大肠杆菌氮利用的调控。
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