定点诱变揭示了保守带电残基对大肠杆菌PheP通透酶转运活性的重要性。
Site-directed mutagenesis reveals the importance of conserved charged residues for the transport activity of the PheP permease of Escherichia coli.
作者信息
Pi J, Wookey P J, Pittard A J
机构信息
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
出版信息
J Bacteriol. 1993 Nov;175(22):7500-4. doi: 10.1128/jb.175.22.7500-7504.1993.
Abstract
Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be essential for transport activity.
摘要
定点诱变已被用于鉴定许多对PheP蛋白转运活性至关重要的带电荷残基。这些残基在氨基酸转运蛋白簇中高度保守。然而，已表明一些其他保守残基和许多芳香族残基对转运活性并非必不可少。
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