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Basis of the superiority of cefoperazone amphotericin teicoplanin for isolating Campylobacter upsaliensis from stools.头孢哌酮、两性霉素、替考拉宁从粪便中分离出犬弯曲杆菌的优势基础。
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本文引用的文献

1
Blood-free selective medium for isolation of Campylobacter jejuni from feces.用于从粪便中分离空肠弯曲菌的无血选择性培养基。
J Clin Microbiol. 1984 Feb;19(2):169-71. doi: 10.1128/jcm.19.2.169-171.1984.
2
Improved blood free selective medium for the isolation of campylobacter jejuni from faecal specimens.用于从粪便标本中分离空肠弯曲菌的改良无血选择性培养基。
J Clin Pathol. 1984 Aug;37(8):956-7. doi: 10.1136/jcp.37.8.956-b.
3
Isolation of Campylobacter: what are we missing?弯曲杆菌的分离：我们遗漏了什么？
J Clin Pathol. 1987 Jun;40(6):702-3. doi: 10.1136/jcp.40.6.702-b.
4
Reassessment of selective agars and filtration techniques for isolation of Campylobacter species from faeces.对用于从粪便中分离弯曲杆菌属细菌的选择性琼脂和过滤技术的重新评估。
Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):155-60. doi: 10.1007/BF01963069.
5
DNA relatedness and biochemical features of Campylobacter spp. isolated in central and South Australia.在澳大利亚中部和南部分离出的弯曲杆菌属的DNA相关性及生化特征
J Clin Microbiol. 1985 Jul;22(1):71-4. doi: 10.1128/jcm.22.1.71-74.1985.
6
Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States.美国与“乌普萨拉弯曲杆菌”（过氧化氢酶阴性或弱阳性弯曲杆菌菌种）相关的人类疾病。
J Clin Microbiol. 1989 Jan;27(1):66-73. doi: 10.1128/jcm.27.1.66-73.1989.
7
Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including "Campylobacter upsaliensis," from humans with gastroenteritis.从患有肠胃炎的人类中分离并鉴定过氧化氢酶阴性和过氧化氢酶弱阳性弯曲杆菌菌株，包括“乌普萨拉弯曲杆菌”。
J Clin Microbiol. 1989 Sep;27(9):2042-5. doi: 10.1128/jcm.27.9.2042-2045.1989.
8
"Campylobacter upsaliensis" isolated from blood cultures of pediatric patients.从儿科患者血培养物中分离出的“乌普萨拉弯曲菌”
J Clin Microbiol. 1989 Apr;27(4):657-9. doi: 10.1128/jcm.27.4.657-659.1989.
9
Characterization and description of "Campylobacter upsaliensis" isolated from human feces.从人类粪便中分离出的“乌普萨拉弯曲菌”的鉴定与描述。
J Clin Microbiol. 1990 May;28(5):1039-46. doi: 10.1128/jcm.28.5.1039-1046.1990.
10
"Campylobacter upsaliensis" sepsis in a boy with acquired hypogammaglobulinemia.一名获得性低丙种球蛋白血症男孩发生“乌普萨拉弯曲杆菌”败血症。
Diagn Microbiol Infect Dis. 1990 Sep-Oct;13(5):367-9. doi: 10.1016/0732-8893(90)90003-e.

用于嗜热弯曲杆菌（包括乌普萨拉弯曲杆菌）的选择性培养基。

Selective medium for thermophilic campylobacters including Campylobacter upsaliensis.

作者信息

Aspinall S T, Wareing D R, Hayward P G, Hutchinson D N

机构信息

Public Health Laboratory, Royal Preston Hospital, Lancs.

出版信息

J Clin Pathol. 1993 Sep;46(9):829-31. doi: 10.1136/jcp.46.9.829.

DOI:10.1136/jcp.46.9.829
PMID:8227433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC501518/
Abstract

AIMS

To develop a selective supplement for the recovery of thermophilic Campylobacter spp, including Campylobacter upsaliensis from faeces, using campylobacter blood free selective agar base as the growth medium.

METHODS

Minimum inhibitory concentrations (MICs) of cefoperazone and of teicoplanin were determined for 51 strains of C upsaliensis, 159 strains of other thermophilic Campylobacter spp, and for 66 Enterobacteriaceae (cefoperazone only). From these results a medium using campylobacter blood free selective agar base incorporating cefoperazone (8 mg/l), amphotericin (10 mg/l), and teicoplanin (4 mg/l)--CAT medium--was formulated and compared with the commercially available campylobacter blood free selective medium (modified CCDA) for isolation of Campylobacter spp from 7000 human faecal specimens. The two media were also compared for the recovery of C upsaliensis from 45 spiked human faeces.

RESULTS

Isolation rates of Campylobacter spp other than C upsaliensis were similar for both media, but the CAT medium alone recovered four of the five strains of C upsaliensis from the faecal samples examined. From the spiked faeces specimens, recovery of C upsaliensis was increased by between 35% and over 200-fold on the CAT medium compared with the modified CCDA.

CONCLUSIONS

CAT selective agar was a suitable alternative medium to modified CCDA for the growth of thermophilic Campylobacter spp, including C upsaliensis from faeces.

摘要

目的

以弯曲菌无血选择性琼脂基础培养基作为生长培养基，开发一种用于从粪便中分离包括乌普萨拉弯曲菌在内的嗜热弯曲菌属的选择性补充培养基。

方法

测定了头孢哌酮和替考拉宁对51株乌普萨拉弯曲菌、159株其他嗜热弯曲菌属菌株以及66株肠杆菌科细菌（仅针对头孢哌酮）的最低抑菌浓度（MIC）。根据这些结果，配制了一种使用含有头孢哌酮（8毫克/升）、两性霉素（10毫克/升）和替考拉宁（4毫克/升）的弯曲菌无血选择性琼脂基础培养基——CAT培养基，并与市售的弯曲菌无血选择性培养基（改良CCDA）进行比较，用于从7000份人类粪便标本中分离弯曲菌属。还比较了这两种培养基从45份加标的人类粪便中回收乌普萨拉弯曲菌的情况。

结果

对于除乌普萨拉弯曲菌外的其他弯曲菌属，两种培养基的分离率相似，但单独使用CAT培养基从所检测的粪便样本中回收了五株乌普萨拉弯曲菌中的四株。与改良CCDA相比，在加标的粪便标本中，CAT培养基上乌普萨拉弯曲菌的回收率提高了35%至200多倍。

结论

对于包括从粪便中分离乌普萨拉弯曲菌在内的嗜热弯曲菌属的生长，CAT选择性琼脂是改良CCDA的合适替代培养基。