Berche P, Véron M, Tinelli R
Ann Microbiol (Paris). 1976 Feb-Mar;127A(2):247-59.
From a strain (72 V) of Pseudomonas aeruginosa, we have prepared an acellular vaccine P2, which showed a good efficiency against homologous experimental infection in mice. The extraction procedure is as follows: washed bacterial cells are suspended in 0,15 M NaCl and heated at 60 degrees centigrade for 1 hr; after centrifugation, the supernatant fluid is precipitated with one and five volumes of ethanol. This acellular vaccine possess the following properties: rapid efficiency (10 days) after a single or three inoculations of very small doses (ED50 = 0.014 mug per mouse); weak toxicity (LD50 = 1,148 mug per mouse, subcutaneous route); capacity for production of specific protective antibodies.
我们从一株铜绿假单胞菌（72 V株）制备了一种无细胞疫苗P2，该疫苗对小鼠的同源实验性感染显示出良好的效果。提取步骤如下：将洗涤后的细菌细胞悬浮于0.15 M氯化钠中，在60摄氏度加热1小时；离心后，用上清液体积1倍和5倍的乙醇进行沉淀。这种无细胞疫苗具有以下特性：单次或三次接种极少量剂量（ED50 = 每只小鼠0.014微克）后起效迅速（10天）；毒性较弱（皮下途径，LD50 = 每只小鼠1148微克）；具有产生特异性保护性抗体的能力。
