de Barjac H, Lecadet M M
C R Acad Hebd Seances Acad Sci D. 1976 Jun 21;282(23):2119-22.
This method can determine amounts of exotoxin near 1 mug. Used on the autoclaved filtrates of cultures of B. thuringiensis var. thuringiensis (serotype 1 type-strain), it shows an excretion rate of 250 to 300 mug per millilitre. The results on different exotoxin-producer strains give a perfect parallelism with the results obtained by bio-assays on Insects, but the biochemical method is more accurate and reproducible. This biochemical technique allows us, for the first time, to place the exotoxin excretion during the sporulation stage and not during the exponential growth.
该方法能够测定接近1微克的外毒素量。用于苏云金芽孢杆菌苏云金变种（血清型1标准菌株）培养物的高压灭菌滤液时，其显示出每毫升250至300微克的排泄率。对不同外毒素产生菌株的检测结果与昆虫生物测定所得结果完全平行，但生化方法更准确且可重复。这种生化技术首次使我们能够确定外毒素排泄发生在芽孢形成阶段而非指数生长阶段。
