Zohri A A, Abdel-Gawad K M
Botany Department, Faculty of Science, Assiut University, Egypt.
J Basic Microbiol. 1993;33(4):279-88. doi: 10.1002/jobm.3620330413.
Fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. Forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-Czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-Czapek's agar medium at 28 +/- 2 degrees C. Penicillium, Aspergillus and cladosporium were the common genera on the two types of media used. Alternaria and Pleospora were common on 1% glucose-Czapek's agar only while the genus of Eurotium was isolated with high occurrence on 40% sucrose-Czapek's agar. The common species on the two types of media used were Penicillium chrysogenum, Aspergillus niger and cladosporium cladosporioides. On 1% glucose-Czapek's agar, P. aurantiogriseum, A. fumigatus, A. flavus, Alternaria tenuissima and Pleospora herbarum were isolated with high occurrence while A. versicolor, A. wentii, Eurotium amstelodami and E. chevalieri were common on 40% sucrose-Czapek's agar. The different dried fruit samples were assayed for the natural occurrence of aflatoxins B1, B2, G1 & G2, citrinin, ochratoxins, patulin, sterigmatocystin, diacetoxyscirpenol, T-2 toxin and zearalenone by thin layer chromatographic analysis. Ochratoxin A was detected in all samples tested of apricot (50-110 micrograms/kg), fig (60-120 micrograms/kg) and plum (210-280 micrograms/kg). The other mycotoxins under investigation were not detected. All samples examined of raisin proved to be mycotoxin free.
从4份干无花果样品以及杏仁、李子和葡萄干各3份干样品中采集并鉴定出属于23个属的55个种和2个变种。在1%葡萄糖察氏培养基上分离出属于20个属的49个种和2个变种,而在40%蔗糖察氏琼脂培养基上,于28±2℃分离出属于16个属的31个种和1个变种。青霉属、曲霉属和枝孢属是所用两种培养基上的常见属。链格孢属和格孢腔菌属仅在1%葡萄糖察氏琼脂上常见,而在40%蔗糖察氏琼脂上,麹霉属的分离率较高。所用两种培养基上的常见种是产黄青霉、黑曲霉和枝状枝孢。在1%葡萄糖察氏琼脂上,橙色灰绿青霉、烟曲霉、黄曲霉、细极链格孢和草本格孢腔菌的分离率较高,而杂色曲霉、温特曲霉、阿姆斯特丹麹霉和谢瓦利埃麹霉在40%蔗糖察氏琼脂上常见。通过薄层色谱分析测定了不同干果样品中黄曲霉毒素B1、B2、G1和G2、桔霉素、赭曲霉毒素、展青霉素、柄曲霉素、二醋酸藨草镰刀菌烯醇、T-2毒素和玉米赤霉烯酮的天然存在情况。在所检测的所有杏仁样品(50 - 110微克/千克)、无花果样品(60 - 120微克/千克)和李子样品(210 - 280微克/千克)中均检测到赭曲霉毒素A。未检测到所研究的其他霉菌毒素。所有检测的葡萄干样品均未检出霉菌毒素。