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埃及空气尘埃颗粒中的真菌区系及产真菌毒素的真菌

Mycoflora and mycotoxin-producing fungi of air-dust particles from Egypt.

作者信息

Abdel-Hafez S I, Shoreit A A, Abdel-Hafez A I, el Maghraby O M

出版信息

Mycopathologia. 1986 Jan;93(1):25-32. doi: 10.1007/BF00437011.

Abstract

Using the dilution-plate method, 27 genera and 64 species were collected from 20 air-dust samples on glucose - (24 genera and 57 species) and cellulose - (21 genera and 45 species) Czapek's agar at 28 degrees C. There are basic similarities between the mycoflora of air-dust on the two media and the most prevalent species were Aspergillus niger, A. flavus, A. ochraceus, A. terreus, A. versicolor, Penicillium chrysogenum, P. funiculosum, Alternaria alternata, Cladosporium herbarum, Fusarium oxysporum, Rhizopus stolonifer and Trichoderma viride. Chaetomium globosum, Stachybotrys chartarum, Humicola grisea and Arthrobotrys oligospora were common only on cellulose agar plates. Extracts of mycelium from 25 isolates were tested with brine schrimp (Artemia salina); of these 23 displayed varying degrees of toxicity. Thin layer chromatographic analysis of 12 isolates of Aspergillus flavus revealed that 4 strains were producing detectable aflatoxin. Zearalenone production was noted for 3 out of 5 strains of Fusarium oxysporum and 2 out of 5 strains of F. solani.

摘要

采用稀释平板法,在28摄氏度下,从20份空气尘埃样本中收集到27个属和64个种,这些样本分别接种于葡萄糖察氏琼脂(24个属和57个种)和纤维素察氏琼脂(21个属和45个种)上。两种培养基上空气尘埃中的真菌区系存在基本相似性,最常见的物种是黑曲霉、黄曲霉、赭曲霉、土曲霉、杂色曲霉、产黄青霉、绳状青霉、链格孢、草本枝孢、尖孢镰刀菌、匍枝根霉和绿色木霉。球毛壳、暗黑穗霉、灰腐质霉和少孢节丛孢仅在纤维素琼脂平板上常见。对25个分离株的菌丝体提取物进行了卤虫(卤虫)测试;其中23个表现出不同程度的毒性。对12个黄曲霉菌株进行薄层色谱分析,发现4个菌株产生可检测到的黄曲霉毒素。5株尖孢镰刀菌中有3株、5株茄病镰刀菌中有2株产生玉米赤霉烯酮。

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