Lu J Z, Sun Y N, Rose R C, Bonnez W, McCance D J
Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642.
J Virol. 1993 Dec;67(12):7131-9. doi: 10.1128/JVI.67.12.7131-7139.1993.
Human papillomaviruses (HPVs) cannot be propagated in vitro, but the DNA can be replicated transiently in an assay in the presence of two trans-acting viral proteins, E1 and E2. Using this assay, we have defined the minimal cis-acting elements of the origin of replication of HPV type 11. Most HPV genomes are conserved at the origin of replication, and the core contains three E2 binding sites (E2BS) surrounding an A/T-rich spacer region. The present results show that the minimal requirement for replication is either two E2BS alone or the A/T-rich region plus one E2BS; in the latter case the relative position of the E2BS is important. In all the studies, the presence of both E1 and E2 proteins was essential for replication, yet only the E2BS was required at the origin. We have shown that E1, E2, and the origin of replication containing an E2BS from a complex in vitro, and our data are consistent with a model in which E2 acts to target E1 to the HPV type 11 replication origin.
人乳头瘤病毒（HPV）无法在体外繁殖，但在两种反式作用病毒蛋白E1和E2存在的情况下，其DNA可在一项检测中短暂复制。利用该检测方法，我们确定了11型HPV复制起点的最小顺式作用元件。大多数HPV基因组在复制起点处是保守的，其核心包含围绕富含A/T间隔区的三个E2结合位点（E2BS）。目前的结果表明，复制的最低要求要么是单独的两个E2BS，要么是富含A/T的区域加上一个E2BS；在后一种情况下，E2BS的相对位置很重要。在所有研究中，E1和E2蛋白的存在对复制至关重要，但在起点处仅需要E2BS。我们已经证明E1、E2以及包含来自体外复合物的E2BS的复制起点，并且我们的数据与一个模型一致，在该模型中E2的作用是将E1靶向到11型HPV复制起点。
