Macnab R M
J Clin Microbiol. 1976 Sep;4(3):258-65. doi: 10.1128/jcm.4.3.258-265.1976.
A method is described for visualizing unstained bacterial flagella by dark-field light microscopy. Since individual filaments can be seen, a genus such as Salmonella, which is peritrichously flagellated, can readily be distinguished from a polarly flagellated genus such as Pseudomonas. Polarly flagellated bacteria generally swim much faster than peritrichously flagellated bacteria, and turn by abrupt reversals. The differences in flagellation and motility provide diagnostic criteria that may be useful in clinical microbiology.
本文描述了一种通过暗视野光学显微镜观察未染色细菌鞭毛的方法。由于可以看到单根鞭毛丝，因此像具有周生鞭毛的沙门氏菌属这样的菌属很容易与像假单胞菌属这样具有极生鞭毛的菌属区分开来。极生鞭毛的细菌通常比周生鞭毛的细菌游动速度快得多，并且通过突然反转来转向。鞭毛着生方式和运动性的差异提供了诊断标准，这在临床微生物学中可能有用。
