磷酸葡萄糖异构酶对α-D-葡萄糖6-磷酸是否具有异头特异性或选择性？通过二维相敏31P交换光谱（EXSY）核磁共振进行评估。
Does phosphoglucoisomerase display anomeric specificity or selectivity towards alpha-D-glucose 6-phosphate? An assessment by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r.
作者信息
Kayser F, Biesemans M, Willem R, Malaisse-Lagae F, Malaisse W J
机构信息
High Resolution NMR Centre, Faculty of Engineering, Free University of Brussels (VUB), Belgium.
出版信息
Biochem J. 1993 Oct 15;295 ( Pt 2)(Pt 2):607-9. doi: 10.1042/bj2950607.
Abstract
The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the enzyme displays anomeric selectivity, rather than specificity, towards alpha-D-glucose 6-phosphate. Indeed, beta-D-glucose 6-phosphate participates for about 20% to the total and direct conversion of the aldohexose into oxohexose ester.
摘要
通过二维相敏31P交换光谱法（EXSY）核磁共振对酵母磷酸葡萄糖异构酶催化反应中己糖6-磷酸酯的相互转化进行研究，结果表明该酶对α-D-葡萄糖6-磷酸酯表现出异头选择性而非特异性。实际上，β-D-葡萄糖6-磷酸酯约占醛己糖向酮己糖酯直接转化总量的20%。
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