Vorobjev V S, Sharonova I N, Walsh I B, Haas H L
Institute of Physiology II, Heinrich-Heine-University, Düsseldorf, Federal Republic of Germany.
Neuron. 1993 Nov;11(5):837-44. doi: 10.1016/0896-6273(93)90113-6.
N-methyl-D-aspartate (NMDA)-evoked currents were recorded from acutely isolated rat hippocampal neurons, using the whole-cell patch-clamp technique and a rapid perfusion system. Histamine, at concentrations from 0.5 to 100 microM, reversibly enhanced NMDA currents by up to 50%. The effect cannot be ascribed to activation of the known histamine receptors (H1, H2, H3) but is occluded by spermine. These results suggest an interaction of histamine with the polyamine-binding site on the NMDA receptor complex. This modulatory action could allow the histaminergic system to determine time and loci of NMDA receptor-mediated events, such as memory formation according to behavioral state.
采用全细胞膜片钳技术和快速灌流系统，从急性分离的大鼠海马神经元记录N-甲基-D-天冬氨酸（NMDA）诱发电流。组胺浓度在0.5至100微摩尔时，可使NMDA电流可逆性增强达50%。该效应不能归因于已知组胺受体（H1、H2、H3）的激活，但可被精胺阻断。这些结果提示组胺与NMDA受体复合物上的多胺结合位点存在相互作用。这种调节作用可能使组胺能系统根据行为状态来确定NMDA受体介导事件（如记忆形成）的时间和位点。
