Amano O, Yoshitake Y, Nishikawa K, Iseki S
Department of Anatomy, Kanazawa University School of Medicine, Japan.
Arch Histol Cytol. 1993 Aug;56(3):269-76. doi: 10.1679/aohc.56.269.
Immunohistochemical localization of basic fibroblast growth factor (bFGF) was studied in the rat pituitary gland at light and electron microscopic levels using a specific monoclonal antibody. In the anterior lobe, bFGF-immunoreactivity was found mostly in a cell population without secretory granules, which was identified as the folliculo-stellate (FS) cell by its ultra-structure and expression of S-100 protein. The subcellular localization of the immunoreactivity in FS cell was strictly nuclear. Besides the FS cell, a minor cell population containing small granules of 60-100 nm diameter, which seemed to represent immature endocrine cells, was immunostained in the nuclei with the anti-bFGF antibody. In the intermediate lobe, a subpopulation of the S-100 protein-positive cells lining the pituitary cleft was immunoreactive for bFGF. These results imply that the agranular cell types are the main producer and/or target of rat pituitary bFGF, and that bFGF may play a role in the differentiation of pituitary endocrine cells.
利用特异性单克隆抗体，在光镜和电镜水平研究了碱性成纤维细胞生长因子（bFGF）在大鼠垂体中的免疫组织化学定位。在前叶，bFGF免疫反应性主要见于一群无分泌颗粒的细胞，根据其超微结构和S-100蛋白表达确定为滤泡星状（FS）细胞。FS细胞中免疫反应性的亚细胞定位严格在细胞核。除FS细胞外，一群含有直径60-100nm小颗粒的较小细胞群，似乎代表未成熟内分泌细胞，用抗bFGF抗体在细胞核中呈免疫染色。在中间叶，垂体裂内衬的S-100蛋白阳性细胞亚群对bFGF呈免疫反应性。这些结果表明无颗粒细胞类型是大鼠垂体bFGF的主要产生者和/或靶细胞，并且bFGF可能在垂体内分泌细胞的分化中起作用。
