Marin F, Boya J
Department of Histology and Cell Biology, Faculty of Medicine, University Complutense, Madrid, Spain.
Neuroendocrinology. 1995 Nov;62(5):523-9. doi: 10.1159/000127043.
The immunocytochemical localization of basic fibroblast growth factor (bFGF) was studied in the human pituitary gland using a polyclonal antibody against fraction 1-24 of bovine recombinant bFGF. From a technical perspective, methacarn-fixed tissues were associated with a better preservation of bFGF immunoreactivity. Basic FGF-immunopositive glandular secretory cells were detected from the fetal period to adulthood in the pars distalis. No bFGF-positive cells were found in the neural lobe, basophil invasion areas, pars tuberalis or the walls of the pituitary cleft in the fetal pituitaries where this area was available. Endothelial cells and the axons of the neurohypophysis appeared weakly immunopositive or immunonegative depending on the fixative. According to their morphology, distribution, and the serial section analysis with all the pituitary hormones and vimentin, a folliculostellate cell marker, we conclude that bFGF-positive cells appear to be somatotropes. These results are consistent with the interpretation that bFGF plays a paracrine role in the modulation of the synthesis and secretion of various pituitary hormones.
利用抗牛重组碱性成纤维细胞生长因子（bFGF）1-24片段的多克隆抗体，研究了人垂体中bFGF的免疫细胞化学定位。从技术角度来看，甲醇-氯仿固定的组织能更好地保存bFGF免疫反应性。在远侧部，从胎儿期到成年期均可检测到碱性FGF免疫阳性的腺分泌细胞。在胎儿垂体有该区域的情况下，在神经叶、嗜碱性细胞浸润区、结节部或垂体裂壁中未发现bFGF阳性细胞。神经垂体的内皮细胞和轴突根据固定剂的不同呈现弱阳性或阴性免疫反应。根据其形态、分布以及与所有垂体激素和波形蛋白（一种滤泡星形细胞标志物）的连续切片分析，我们得出结论，bFGF阳性细胞似乎是生长激素细胞。这些结果与bFGF在调节各种垂体激素的合成和分泌中起旁分泌作用的解释一致。
