通过起始抑制剂在体内使多核糖体解体后，信使核糖核酸保留在内质网膜上。
Retention of mRNA on the endoplasmic reticulum membranes after in vivo disassembly of polysomes by an inhibitor of initiation.
作者信息
Adesnik M, Lande M, Martin T, Sabatini D D
出版信息
J Cell Biol. 1976 Oct;71(1):307-13. doi: 10.1083/jcb.71.1.307.
Abstract
Membrane-bound ribosomes and messenger RNA remained associated with the microsomal membranes of human fibroblasts after cultures were treated with Verrucarin A, an inhibitor of initiation which led to extensive run-off of ribosomes from polysomal structures. When a membrane fraction from Verrucarin-treated cells containing such inactive ribosomes and mRNA was suspended in a medium of high salt concentration, extensive release of ribosomal subunits occurred without the need for puromycin. The mRNA nevertheless remained associated with the membranes. These results add support to the conclusion that, in human fibroblasts, mRNA is bound directly to ER membranes, independently of the ribosomes and nascent polypeptide chains.
摘要
在用疣孢菌素A（一种起始抑制剂，可导致核糖体从多核糖体结构大量解离）处理培养物后，人成纤维细胞的膜结合核糖体和信使核糖核酸仍与微粒体膜结合。当将来自经疣孢菌素处理的细胞、含有这种无活性核糖体和信使核糖核酸的膜部分悬浮于高盐浓度培养基中时，核糖体亚基大量释放，且无需嘌呤霉素。然而，信使核糖核酸仍与膜结合。这些结果支持了这样的结论：在人成纤维细胞中，信使核糖核酸直接与内质网膜结合，独立于核糖体和新生多肽链。
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