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利用农业废弃物通过固态发酵优化蛋白酶的生产。

Optimization of protease production from under solid state fermentation using agrowastes.

作者信息

Balachandran Chellapandian, Vishali Alagumalai, Nagendran Natarajan Arun, Baskar Kathirvelu, Hashem Abeer, Abd Allah Elsayed Fathi

机构信息

Department of Biotechnology, Thiagarajar College, Madurai 625 009, Tamil Nadu, India.

National Centre of Excellence (MHRD), Thiagarajar College, Madurai 625 009, Tamil Nadu, India.

出版信息

Saudi J Biol Sci. 2021 Aug;28(8):4263-4269. doi: 10.1016/j.sjbs.2021.04.069. Epub 2021 May 1.

Abstract

Marine microbes are potential source for novel metabolites. They are efficient in producing these metabolites utilizing agrowastes. Protease is one of the enzymes which find wide industrial applications. In the present study, protease producing bacteria was isolated from marine sediments and the organism was identified as The organism was subjected to protease production under solid state fermentation (SSF) using different agrowastes as substrates. Among the substrates used, wheat bran yielded maximum quantity of protease. The fermentation process was carried out under different cultural conditions to optimize the parameters influencing the enzyme production. The results of the stain removal studies by the enzyme revealed the increased efficiency of the microbial enzyme than the commercial detergent.

摘要

海洋微生物是新型代谢产物的潜在来源。它们利用农业废弃物高效生产这些代谢产物。蛋白酶是一种具有广泛工业应用的酶。在本研究中,从海洋沉积物中分离出产蛋白酶的细菌,并将该菌株鉴定为 。使用不同的农业废弃物作为底物,通过固态发酵(SSF)对该菌株进行蛋白酶生产。在所使用的底物中,麦麸产生的蛋白酶量最大。在不同的培养条件下进行发酵过程,以优化影响酶生产的参数。酶的去污研究结果表明,微生物酶的效率高于市售洗涤剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/982d/8324923/ecfcc38594bc/gr1.jpg

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