Stackebrandt E, Sproer C, Rainey F A, Burghardt J, Päuker O, Hippe H
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Int J Syst Bacteriol. 1997 Oct;47(4):1134-9. doi: 10.1099/00207713-47-4-1134.
Almost complete 16S ribosomal DNA (rDNA) sequences were determined for the type strains of nine species belonging to the genus Desulfotomaculum and for seven strains described as strains of this genus. The sequences were compared with previously published 16S rDNA and rRNA sequences of the type strains of the other species of the genus. The majority of the species form a phylogenetically coherent cluster within the Clostridium-Bacillus subphylum of gram-positive bacteria. The cluster consists of phylogenetically well-separated lineages containing (i) Desulfotomaculum nigrificans, Desulfotomaculum aeronauticum, and Desulfotomaculum ruminis, (ii) Desulfotomaculum geothermicum, Desulfotomaculum thermosapovorans, and Desulfotomaculum sapomandens, (iii) Desulfotomaculum kuznetsovii, Desulfotomaculum australicum, and Desulfotomaculum thermocisternum, (iv) Desulfotomaculum thermobenzoicum and Desulfotomaculum thermoacetoxidans, and (v) Desulfotomaculum acetoxidans. Some as-yet-undescribed Desulfotomaculum strains are phylogenetically well-separated from strains of the described species. Desulfotomaculum guttoideum shares extremely high 16S rDNA similarity with certain Clostridium species (e.g., Clostridium sphenoides and Clostridium celerecrescens) and is most likely a misidentified species. Desulfotomaculum orientis represents a new genus which branches most closely to the genus Desulfitobacterium. The name Desulfosporosinus orientis gen. nov., comb. nov., is proposed for this taxon.
测定了脱硫肠状菌属9个种的模式菌株以及7株被描述为该属菌株的近乎完整的16S核糖体DNA（rDNA）序列。将这些序列与该属其他种的模式菌株先前发表的16S rDNA和rRNA序列进行了比较。大多数种在革兰氏阳性菌的梭菌-芽孢杆菌亚门内形成一个系统发育上连贯的簇。该簇由系统发育上分隔良好的谱系组成，包括（i）黑色脱硫肠状菌、嗜气脱硫肠状菌和反刍脱硫肠状菌，（ii）地热脱硫肠状菌、嗜热糖分解脱硫肠状菌和食甘露糖脱硫肠状菌，（iii）库兹涅佐夫脱硫肠状菌、澳大利亚脱硫肠状菌和热池脱硫肠状菌，（iv）热苯甲酸脱硫肠状菌和热乙酰氧化脱硫肠状菌，以及（v）乙酰氧化脱硫肠状菌。一些尚未描述的脱硫肠状菌菌株在系统发育上与已描述种的菌株分隔良好。滴状脱硫肠状菌与某些梭菌属物种（如楔形梭菌和快速生长梭菌）具有极高的16S rDNA相似性，很可能是一个被错误鉴定的物种。东方脱硫肠状菌代表一个新属，它与脱硫脱硫弧菌属的亲缘关系最为密切。为此分类单元提出新属名东方脱硫孢窦菌属（Desulfosporosinus orientis gen. nov.），新组合名（comb. nov.）。
