Janssen D, De Rycke P H, Osuna A
Laboratorium voor Zoöfysiologie, Universiteit Gent, Belgium.
Folia Parasitol (Praha). 1993;40(2):109-13.
Hydatid fluid (HF) from hydatid cysts of the cestode Echinococcus granulosus inhibited the phagocytosis of bacteria and yeast cells by host macrophages in vitro. Different assays were used to study the dose-dependent effect of partially purified HF toxins on peritoneal macrophages (PM phi) of the mouse. Trypan blue exclusion, as well as measuring the specific release of lactate dehydrogenase activity and 51Cr release of 51Cr-labelled PM phi, showed that incubation with HF toxins lead to the lysis of the target cells. By measuring the effect of the toxins on the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide by PM phi, and by experimental labelling using [3H]-uridine, a decrease in metabolic activity was seen. However, at non-lytic concentrations, the PM phi showed a peak of metabolic activity. The observations of the effects on macrophages by parasite-derived toxins may be related to a mechanism by which the parasite survives within an immunized host.
来自细粒棘球绦虫包虫囊肿的囊液（HF）在体外可抑制宿主巨噬细胞对细菌和酵母细胞的吞噬作用。采用不同的检测方法研究了部分纯化的HF毒素对小鼠腹腔巨噬细胞（PM phi）的剂量依赖性效应。台盼蓝排斥试验以及测量乳酸脱氢酶活性的特异性释放和51Cr标记的PM phi的51Cr释放，结果表明与HF毒素孵育会导致靶细胞裂解。通过测量毒素对PM phi还原3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐的影响，以及使用[3H]-尿苷进行实验标记，发现代谢活性降低。然而，在非裂解浓度下，PM phi表现出代谢活性峰值。寄生虫衍生毒素对巨噬细胞影响的观察结果可能与寄生虫在免疫宿主体内存活的机制有关。
