Djabali K, Zissopoulou A, de Hoop M J, Georgatos S D, Dotti C G
Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.
J Cell Biol. 1993 Dec;123(5):1197-206. doi: 10.1083/jcb.123.5.1197.
Previous studies have shown that neuronal cells in culture can switch neurotransmitters when grown in the presence of different target cells. To examine whether this plasticity extends to structural proteins, we cocultured hippocampal neurons and pituitary-derived neuroendocrine (AtT20) cells with astrocytes, kidney epithelial cells, or skeletal muscle cells. As a marker of phenotypic change we used the cytoskeletal protein peripherin, a type III intermediate filament (IF) subunit which is not expressed in hippocampal neurons and AtT20 cells. We show here that soluble factor(s) secreted specifically from skeletal muscle cells can induce the expression and de novo assembly of peripherin in a subset of post-mitotic neurons. We further demonstrate that one of these factors is the Leukemia Inhibitory Factor/Cholinergic Neuronal Differentiation Factor. The environmentally regulated expression of peripherin implies a remarkable degree of plasticity in the cytoskeletal organization of postmitotic CNS cells and provides a noninvasive model system to examine the de novo assembly of IF proteins under in vivo conditions.
以往的研究表明，培养中的神经元细胞在与不同靶细胞共同培养时可以转换神经递质。为了研究这种可塑性是否扩展到结构蛋白，我们将海马神经元和垂体来源的神经内分泌（AtT20）细胞与星形胶质细胞、肾上皮细胞或骨骼肌细胞共同培养。作为表型变化的标志物，我们使用细胞骨架蛋白外周蛋白，它是一种III型中间丝（IF）亚基，在海马神经元和AtT20细胞中不表达。我们在此表明，骨骼肌细胞特异性分泌的可溶性因子可诱导有丝分裂后神经元亚群中外周蛋白的表达和从头组装。我们进一步证明，其中一种因子是白血病抑制因子/胆碱能神经元分化因子。外周蛋白的环境调节表达意味着有丝分裂后中枢神经系统细胞的细胞骨架组织具有显著程度的可塑性，并提供了一个非侵入性模型系统来研究体内条件下IF蛋白的从头组装。
