The isolation and characterization of camel (Camelus dromedarius) immunoglobulin classes and subclasses.

Immunoglobulins G and M were prepared from camel sera by ammonium sulphate precipitation, gel filtration and fast protein liquid chromatography (ion exchange). Heavy and light chains were identified by polyacrylamide gel electrophoresis and Western blotting. IgG subclasses were isolated by DEAE ion exchange chromatography and shown to have different electrophoretic motilities. No molecules corresponding to IgA were detected. Rabbit anti-serum against camel IgG was generated and its specificity shown by Western blotting.