Moutaouakil M, Monod M, Prévost M C, Bouchara J P, Paris S, Latgé J P
Unité de Mycologie, Institut Pasteur, Paris, France.
J Med Microbiol. 1993 Nov;39(5):393-9. doi: 10.1099/00222615-39-5-393.
Aspergillus fumigatus produced a 33-kDa serine protease (ALP) in vitro and in vivo. In vitro, this alkaline protease was secreted when the fungus was cultivated in the absence of protein, if the pH of the medium remained close to neutrality. Western blotting and immunoelectronmicroscopy studies showed that ALP was localised in the wall of the fungus and was degraded after secretion in the culture medium under conditions of low pH. Although present in the lung during infection, ALP did not appear to be diagnostically useful and was different from the precipitating chymotrypsin antigen used in the diagnosis of aspergilloma.
烟曲霉在体外和体内均产生一种33 kDa的丝氨酸蛋白酶（ALP）。在体外，如果培养基的pH值保持接近中性，当真菌在无蛋白质的情况下培养时，这种碱性蛋白酶会被分泌出来。蛋白质印迹法和免疫电子显微镜研究表明，ALP定位于真菌细胞壁，在低pH条件下分泌到培养基中后会被降解。尽管在感染期间肺中存在ALP，但它似乎没有诊断价值，并且与用于曲菌球诊断的沉淀性糜蛋白酶抗原不同。
