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一种具有类Rev反应元件特性的新型乙型肝炎病毒（HBV）遗传元件，它对于HBV基因产物的表达至关重要。

A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products.

作者信息

Huang J, Liang T J

机构信息

Gastrointestinal Unit, Massachusetts General Hospital, Boston.

出版信息

Mol Cell Biol. 1993 Dec;13(12):7476-86. doi: 10.1128/mcb.13.12.7476-7486.1993.

DOI:10.1128/mcb.13.12.7476-7486.1993
PMID:8246965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC364819/
Abstract

Many viruses possess complex mechanisms involving multiple gene products and cis-regulatory elements in order to achieve a fine control of their gene expression at both transcriptional and posttranscriptional levels. Hepatitis B virus (HBV) and retroviruses share many structural and functional similarities. In this study, by genetic and biochemical analyses, we have demonstrated the existence of a novel genetic element within the HBV genome which is essential for high-level expression of viral gene products. This element is located 3' to the envelope coding region. We have shown that this genetic element is cis acting at the posttranscriptional level and that its function is exerted at the level of RNA processing as part of transcribed sequences. This RNA element is also functional in the context of a heterologous gene. Similar to the function of Rev-Rev response element interaction of human immunodeficiency virus type 1, this element appears to inhibit the splicing process and facilitate the transport and utilization of HBV transcripts.

摘要

许多病毒拥有复杂的机制，涉及多种基因产物和顺式调控元件，以便在转录和转录后水平上对其基因表达进行精细控制。乙型肝炎病毒（HBV）和逆转录病毒在结构和功能上有许多相似之处。在本研究中，通过遗传学和生物化学分析，我们证明了HBV基因组中存在一种新型遗传元件，它对于病毒基因产物的高水平表达至关重要。该元件位于包膜编码区的3'端。我们已经表明，这种遗传元件在转录后水平上是顺式作用的，并且其功能在RNA加工水平上作为转录序列的一部分发挥作用。这种RNA元件在异源基因的背景下也具有功能。类似于人类免疫缺陷病毒1型的Rev-Rev反应元件相互作用的功能，该元件似乎抑制剪接过程，并促进HBV转录本的运输和利用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/02db3bf97e0d/molcellb00024-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/000f5003a838/molcellb00024-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/729f9b5700ff/molcellb00024-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/346e6a93fc7d/molcellb00024-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/ac8e60718ac2/molcellb00024-0294-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/caef2535ae71/molcellb00024-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/b1151485e6b2/molcellb00024-0295-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/02db3bf97e0d/molcellb00024-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/000f5003a838/molcellb00024-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/729f9b5700ff/molcellb00024-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/346e6a93fc7d/molcellb00024-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/ac8e60718ac2/molcellb00024-0294-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/caef2535ae71/molcellb00024-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/b1151485e6b2/molcellb00024-0295-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a7/364819/02db3bf97e0d/molcellb00024-0296-a.jpg

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