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染色质结构与真核调控基因GAL80的调控

Chromatin structure and regulation of the eukaryotic regulatory gene GAL80.

作者信息

Lohr D

机构信息

Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287-1604.

出版信息

Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10628-32. doi: 10.1073/pnas.90.22.10628.

DOI:10.1073/pnas.90.22.10628
PMID:8248154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC47830/
Abstract

The chromatin structure around the 5' end of the yeast regulatory gene GAL80 has been determined. The chromatin organization is very similar to that on the 5' regions of the GAL1-10 structural genes: a constitutive hypersensitive region containing the upstream activating sequence (UAS) element, and nucleosomes around this hypersensitive region. The downstream nucleosome, which is a positioned nucleosome, covers the TATA and transcription start sites. The nucleosome upstream of the hypersensitive region undergoes significant change when cells are grown in galactose, where GAL80 gene expression is induced to maximal levels. The change may be related to the induction process. GAL4 protein binds strongly to the GAL80 UAS in galactose-grown cells, less strongly in glycerol-grown cells, and not at all in glucose-grown cells. These data and published gene expression data are used to develop a model for the regulation of the GAL80 regulatory gene.

摘要

酵母调控基因GAL80 5'端周围的染色质结构已被确定。其染色质组织与GAL1 - 10结构基因5'区域的非常相似：一个包含上游激活序列（UAS）元件的组成型超敏区域，以及围绕该超敏区域的核小体。下游核小体是一个定位核小体，覆盖TATA和转录起始位点。当细胞在半乳糖中生长时，超敏区域上游的核小体发生显著变化，此时GAL80基因表达被诱导至最高水平。这种变化可能与诱导过程有关。GAL4蛋白在半乳糖培养的细胞中与GAL80 UAS强烈结合，在甘油培养的细胞中结合较弱，而在葡萄糖培养的细胞中完全不结合。这些数据和已发表的基因表达数据被用于建立一个GAL80调控基因的调控模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/990d2fd9b87e/pnas01529-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/c10ad5fede22/pnas01529-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/0347e74660a4/pnas01529-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/990d2fd9b87e/pnas01529-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/c10ad5fede22/pnas01529-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/0347e74660a4/pnas01529-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37bd/47830/990d2fd9b87e/pnas01529-0229-a.jpg

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Chromatin structure and regulation of the eukaryotic regulatory gene GAL80.染色质结构与真核调控基因GAL80的调控
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本文引用的文献

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The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
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Multiple protein-DNA interactions over the yeast HSC82 heat shock gene promoter.酵母HSC82热休克基因启动子上的多种蛋白质 - DNA相互作用。
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Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.酵母转录正向调节因子GAL4基因的分离与初步鉴定。
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Mol Cell Biol. 1984 Aug;4(8):1521-7. doi: 10.1128/mcb.4.8.1521-1527.1984.