[质粒pSVc-myc-1在培养的中国仓鼠细胞中诱导基因突变和染色体畸变]
[Plasmid pSVc-myc-1 induces gene mutations and chromosome aberrations in cultured Chinese hamster cells].
作者信息
Bobrysheva I V, Baron E M, Varshaver N B
出版信息
Tsitol Genet. 1993 Jul-Aug;27(4):51-5.
The induction of mutations to 6-thioguanine-resistance and chromosome aberrations by the plasmid pSVc-myc-1, carrying the activated cellular oncogene c-myc, isolated from a mouse plasmocytoma was studied in a cultured Chinese hamster cell line. The yield of HPRT- mutants and chromosome aberrations increased 1.6 times on the average after pSVc-myc-1 treatment. The mutagenic activity of pSVc-myc-1 was statistically significant. The role of mutagenic effects of activated cellular oncogenes in malignant transformations is discussed.
对从一只小鼠浆细胞瘤中分离出的携带激活型细胞癌基因c-myc的质粒pSVc-myc-1，在培养的中国仓鼠细胞系中诱导产生对6-硫鸟嘌呤抗性的突变以及染色体畸变的情况进行了研究。经pSVc-myc-1处理后，次黄嘌呤磷酸核糖基转移酶（HPRT）突变体的产量和染色体畸变平均增加了1.6倍。pSVc-myc-1的诱变活性具有统计学意义。文中讨论了激活型细胞癌基因的诱变作用在恶性转化中的作用。
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