Rubashevskiĭ E L, Patskovskiĭ Iu V, Podol'skaia S V, Sukhorada E M, Lysenko E F, Kostetskaia E V, Kostetskiĭ I E, Varzanova I S, Lukash L L
Tsitol Genet. 1993 May-Jun;27(3):63-8.
The mutagenic activity of the pUC19 bacterial plasmid DNA and the pAins recombinant plasmid DNA carrying human insulin gene has been investigated. Both pUC19 and pAins plasmid DNAs have been shown to induce the gene mutations in hprt locus of Chinese hamster cell line. The high level of the gene mutations (similar to the indices of the gene mutations induced by the chemical mutagens) has been in the focus of attention. The conclusion has been made concerning impossibility to use pAins plasmid DNA in the diabetes mellitus gene therapy. It is necessary to test the mutagenic properties of the DNA molecules produced for gene therapy of human inherited diseases.
已对pUC19细菌质粒DNA和携带人胰岛素基因的pAins重组质粒DNA的诱变活性进行了研究。已证明pUC19和pAins质粒DNA均可诱导中国仓鼠细胞系hprt位点的基因突变。高水平的基因突变（类似于化学诱变剂诱导的基因突变指数）一直是关注的焦点。得出了关于不能将pAins质粒DNA用于糖尿病基因治疗的结论。有必要对用于人类遗传病基因治疗所产生的DNA分子的诱变特性进行检测。
