Pollesello P, Toffanin R, Eriksson O, Kilpeläinen I, Hynninen P H, Paoletti S, Saris N E
Department of Biochemistry, Biophysics, and Macromolecular Chemistry, University of Trieste, Italy.
Anal Biochem. 1993 Oct;214(1):238-44. doi: 10.1006/abio.1993.1483.
Phospho- and glycolipids with mono- or polyunsaturated fatty acid chains, free and acylated steroids, carotenes and carotenoids, chlorophylls, and related pheophytins were detected in crude extracts by NMR spectroscopy without any need for prior separation. A broad range of molecules belonging to different lipid classes could be identified in one-dimensional 13C NMR spectra obtained by applying a polarization transfer pulse sequence (DEPT 135 degrees). Directly detected or 1H-detected two-dimensional heterocorrelated NMR experiments were performed to facilitate the assignment of peaks arising from carotenoids, unsaturated fatty acid chains, and chlorophylls. 13C NMR data of crude lipid extracts from the macroalagae Ulva rigida and Fucus virsoides are shown to yield an informative overview of their lipid content. NMR is thus proposed as a simple, nonselective, and nondestructive technique for the first screening of the main lipid classes in complex lipid mixtures.
通过核磁共振光谱法在粗提物中检测到了带有单不饱和或多不饱和脂肪酸链的磷酸脂和糖脂、游离及酰化类固醇、类胡萝卜素和类胡萝卜素酯、叶绿素以及相关的脱镁叶绿素，无需事先进行分离。通过应用极化转移脉冲序列（DEPT 135度）获得的一维碳-13核磁共振谱中，可以鉴定出属于不同脂质类别的多种分子。进行直接检测或氢检测的二维异核相关核磁共振实验，以促进对类胡萝卜素、不饱和脂肪酸链和叶绿素产生的峰的归属。来自大型海藻石莼和墨角藻的粗脂质提取物的碳-13核磁共振数据显示，能够提供其脂质含量的信息概况。因此，核磁共振被提议作为一种简单、非选择性且无损的技术，用于对复杂脂质混合物中的主要脂质类别进行初步筛选。
