鼠伤寒沙门氏菌中ATP特异性消耗方法的比较。
Comparison of methods for specific depletion of ATP in Salmonella typhimurium.
作者信息
Johnson M S, Taylor B L
机构信息
Department of Microbiology, Loma Linda University School of Medicine, California 92350.
出版信息
Appl Environ Microbiol. 1993 Oct;59(10):3509-12. doi: 10.1128/aem.59.10.3509-3512.1993.
Abstract
Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.
摘要
对鼠伤寒沙门氏菌中三种ATP耗竭方法进行了比较。砷酸盐处理后ATP浓度最低。砷酸盐或α-甲基葡萄糖苷加叠氮化物处理非特异性地降低了所有三磷酸核苷酸水平。hisF突变体中的组氨酸饥饿对ATP耗竭具有相对特异性，因此在区分ATP依赖性过程与其他核苷酸依赖性过程方面具有潜力。
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