Kondo Y, Takano F, Hojo H
Biochem Pharmacol. 1993 Nov 17;46(10):1861-3. doi: 10.1016/0006-2952(93)90594-m.
The bisbenzylisoquinoline (BBI) alkaloids chondocurine, cycleanine, tetrandrine and berbamine were tested for their capacity to suppress hepatic injury and production of tumor necrosis factor (TNF) induced by lipopolysaccharide (LPS) in mice primed with bacillus Calmette-Guerin (BCG). When administered for three consecutive days before LPS injection, chondocurine, cycleanine and tetrandrine (10 mg/kg/day) strongly suppressed serum alanine aminotransferase (EC 2.6.1.1.) and aspartate aminotransferase (EC 2.6.1.2.); however, berbamine gave only slight protection. Chondocurine, cycleanine and tetrandrine but not berbamine significantly reduced the level of TNF which peaked 2 hr after LPS injection. This study shows that BBI alkaloids prevent BCG/LPS-induced hepatitis at least in part by suppressing TNF production.
对双苄基异喹啉（BBI）生物碱软骨豆碱、环轮宁、粉防己碱和小檗胺进行了测试，以考察它们抑制卡介苗（BCG）致敏小鼠中脂多糖（LPS）诱导的肝损伤及肿瘤坏死因子（TNF）产生的能力。在注射LPS前连续三天给药时，软骨豆碱、环轮宁和粉防己碱（10毫克/千克/天）能强烈抑制血清丙氨酸转氨酶（EC 2.6.1.1.）和天冬氨酸转氨酶（EC 2.6.1.2.）；然而，小檗胺仅提供轻微保护作用。软骨豆碱、环轮宁和粉防己碱而非小檗胺能显著降低LPS注射后2小时达到峰值的TNF水平。本研究表明，BBI生物碱至少部分地通过抑制TNF产生来预防BCG/LPS诱导的肝炎。
