Otsuka F, Chi H I, Umebayashi Y
Department of Dermatology, University of Tsukuba, Japan.
Clin Exp Dermatol. 1993 Sep;18(5):421-4. doi: 10.1111/j.1365-2230.1993.tb02241.x.
Two cases of presumed Spitz naevus, whose diagnosis on clinical and histological grounds was uncertain, were examined for cellular DNA content using the technique of DAPI-DNA microfluorometry. They were compared with 20 cases, respectively, of clinically and histologically confirmed, Spitz naevus, malignant melanoma and acquired pigmented naevus. The two Spitz naevi showed a diploid pattern in a distribution histogram of cellular DNA content. The pattern was similar to that of confirmed Spitz naevi and of acquired pigmented naevi but different from the aneuploid pattern of malignant melanomas. DNA index values of the two cases were within the range of confirmed Spitz naevi and different from those of malignant melanomas. The DAPI-DNA microfluorometric method thus provided confirmatory evidence for the diagnosis of Spitz naevus. The method appears to reflect sensitively the biological behaviour of tumour cells, and is a useful aid to the diagnosis of uncertain Spitz naevi.
两例临床和组织学诊断不明确的疑似斯皮茨痣患者，采用DAPI-DNA显微荧光测定技术检测细胞DNA含量。分别与20例临床和组织学确诊的斯皮茨痣、恶性黑色素瘤及获得性色素痣进行比较。这两例斯皮茨痣在细胞DNA含量分布直方图中显示为二倍体模式。该模式与确诊的斯皮茨痣及获得性色素痣相似，但与恶性黑色素瘤的非整倍体模式不同。这两例的DNA指数值在确诊的斯皮茨痣范围内，与恶性黑色素瘤不同。因此，DAPI-DNA显微荧光测定法为斯皮茨痣的诊断提供了确证依据。该方法似乎能敏感地反映肿瘤细胞的生物学行为，对诊断不明确的斯皮茨痣有一定帮助。
