Umebayashi Y, Otsuka F
Department of Dermatology, University of Tsukuba, Ibaraki, Japan.
Arch Dermatol Res. 1995;287(8):718-22. doi: 10.1007/BF01105795.
The nuclear DNA content of 47 primary malignant melanomas was measured by 4',6-diamidino-2-phenylindole-DNA (DAPI-DNA) microfluorimetry, and the DNA index, a quantitative measure of nuclear DNA content, was calculated. The DNA index and other clinical and pathological variables were examined and compared with patient survival using univariate and multivariate analyses. The Kaplan-Meier life table method revealed that the DNA index and metastases to regional lymph nodes significantly correlated with patient survival. A Cox proportional hazards multivariate analysis demonstrated that the DNA index, which was not significantly correlated with other variables, was the most reliable and independent factor for predicting patient survival.
通过4',6-二脒基-2-苯基吲哚-DNA（DAPI-DNA）显微荧光测定法测量了47例原发性恶性黑色素瘤的核DNA含量，并计算了DNA指数，它是核DNA含量的一种定量测量方法。采用单因素和多因素分析，对DNA指数及其他临床和病理变量进行了检测，并与患者生存率进行了比较。Kaplan-Meier生存表法显示，DNA指数和区域淋巴结转移与患者生存率显著相关。Cox比例风险多因素分析表明，DNA指数与其他变量无显著相关性，是预测患者生存率最可靠、最独立的因素。
