[蓝色葡聚糖的发色团：一种用于通过亲和色谱法纯化干扰素的强效配体]
[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].
作者信息
de Maeyer-Guignard J, de Maeyer E
出版信息
C R Acad Hebd Seances Acad Sci D. 1976 Sep 27;283(6):709-11.
PMID:825322
Abstract
The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.
摘要
蓝色葡聚糖的发色团可作为干扰素亲和层析的配体。将粗制干扰素制剂通过蓝色葡聚糖 - 琼脂糖柱一次可去除90%以上的污染蛋白。用高摩尔浓度的水性缓冲液洗脱可得到比活性高达每毫克蛋白质4.5×10⁶单位的干扰素制剂，回收率为100%。
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