Martinazzi M, Crivelli F, Zampatti C, Martinazzi S
Postgraduate School of Anatomical Pathology, University of Pavia, Italy.
J Clin Pathol. 1993 Nov;46(11):1009-10. doi: 10.1136/jcp.46.11.1009.
To determine the immunohistochemical expression of epidermal growth factor receptor (EGF-R) in high grade, intermediate, and low grade tumours.
Specimens from 931 breast carcinomas were partly formalin fixed and paraffin wax embedded, to classify cases, and partly frozen in liquid nitrogen, cryostat sectioned, and immunostained using two monoclonal antibodies from clone 455 and 528 to demonstrate EGF-R positive cells. An avidin-biotin complex and peroxidase method was used after incubation with biotinylated anti-mouse antibody; colour was developed using a diaiminobenzidine solution.
Low grade carcinomas seldom expressed EGF-R (n = 3) compared with 106 high grade infiltrating ductal carcinomas: EGF-R positive cases were much less common in infiltrating lobular than in infiltrating ductal carcinoma. Medullary carcinomas did not differ from infiltrating ductal carcinomas.
The very low incidence of EGF-R positive cases in the "special type" group of breast carcinomas with a good prognosis is in line with the absence of the homologous c-erbB-2 and p53 oncoproteins, and the rarity of highly proliferating and oestrogen/progesterone negative cases. EGF-R expression in infiltrating lobular carcinoma was in keeping with the intermediate behaviour of this kind of tumour. EGF-R expression in cases of pure medullary carcinoma is the same as that of high grade tumours.
确定表皮生长因子受体（EGF-R）在高分化、中分化和低分化肿瘤中的免疫组化表达情况。
取自931例乳腺癌的标本一部分用福尔马林固定并石蜡包埋用于病例分类，另一部分在液氮中冷冻，冰冻切片，并用克隆号为455和528的两种单克隆抗体进行免疫染色以显示EGF-R阳性细胞。与生物素化抗小鼠抗体孵育后采用抗生物素蛋白-生物素复合物和过氧化物酶方法；用二氨基联苯胺溶液显色。
与106例高分化浸润性导管癌相比，低分化癌很少表达EGF-R（n = 3）；浸润性小叶癌中EGF-R阳性病例比浸润性导管癌少见得多。髓样癌与浸润性导管癌无差异。
预后良好的乳腺癌“特殊类型”组中EGF-R阳性病例的发生率极低，这与同源c-erbB-2和p53癌蛋白的缺失以及高增殖性和雌激素/孕激素阴性病例的罕见情况相符。浸润性小叶癌中的EGF-R表达与这类肿瘤的中间行为一致。纯髓样癌病例中的EGF-R表达与高分化肿瘤相同。
