Gwon A, Mantras C, Gruber L, Cunanan C
Allergan Pharmaceuticals, Irvine, California 92713-9534.
Invest Ophthalmol Vis Sci. 1993 Dec;34(13):3483-8.
To evaluate the effect of Concanavalin A (Con A) on cataract formation in New Zealand Albino rabbits. Uveitis is a chronic inflammatory condition of the eye involving the anterior and/or posterior segments. It may be acute or chronic and is associated with the development of posterior subscapular cataract over time. Con A is a nonspecific inflammatory agent and mitogen for T cells and some B cells. Used extensively in immunogenic studies Con A has been shown to induce uveitis after intravitreal injection in New Zealand Albino rabbits.
In two separate studies, Con A was injected intracamerally or intravitreally into one eye of 12 New Zealand Albino rabbits and an equal volume of balanced salt solution was injected into the opposite eye as a control. In a third study, the effect of topical steroids after intravitreal injection of Con A was evaluated. In all studies, anterior and posterior inflammation and the development of cataract was monitored by slit lamp biomicroscopy and photography. Cataract formation was also studied histopathologically.
Initially, all eyes treated with Con A demonstrated moderate anterior chamber inflammation while eyes treated with balanced salt solution showed no inflammation. Three months after treatment, posterior subcapsular cataracts were present in all rabbit eyes treated with intravitreal Con A. In the third study, topical steroid treatment of Con A-induced inflammation significantly reduced anterior chamber inflammation but had no effect on vitreous humor and posterior subcapsular cataract formation.
This experimental model was the first to demonstrate the development of posterior subcapsular cataracts after Con-A induced inflammation. The cataract was clinically and histologically similar to human posterior subscapular cataracts.
评估伴刀豆球蛋白A（Con A）对新西兰白兔白内障形成的影响。葡萄膜炎是一种累及眼前段和/或眼后段的眼部慢性炎症性疾病。它可以是急性或慢性的，并且随着时间的推移与后囊下白内障的发展有关。Con A是一种非特异性炎症因子，是T细胞和一些B细胞的丝裂原。Con A在免疫原性研究中被广泛使用，已证明在新西兰白兔玻璃体内注射后可诱发葡萄膜炎。
在两项独立研究中，将Con A经前房或玻璃体内注射到12只新西兰白兔的一只眼睛中，并将等量的平衡盐溶液注射到对侧眼睛作为对照。在第三项研究中，评估了玻璃体内注射Con A后局部使用类固醇的效果。在所有研究中，通过裂隙灯生物显微镜检查和摄影监测前、后段炎症以及白内障的发展。还对白内障形成进行了组织病理学研究。
最初，所有接受Con A治疗的眼睛均表现出中度前房炎症，而接受平衡盐溶液治疗的眼睛未出现炎症。治疗三个月后，所有接受玻璃体内Con A治疗的兔眼均出现后囊下白内障。在第三项研究中，局部使用类固醇治疗Con A诱导的炎症可显著减轻前房炎症，但对玻璃体和后囊下白内障形成没有影响。
该实验模型首次证明了Con A诱导炎症后后囊下白内障的发生。该白内障在临床和组织学上与人类后囊下白内障相似。
