增殖性糖尿病视网膜病变的视网膜前膜：一项免疫组织化学研究。

Epiretinal membrane of proliferative diabetic retinopathy: an immunohistochemical study.

作者信息

Hosoda Y, Okada M, Matsumura M, Ogino N, Honda Y, Nagai Y

机构信息

Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.

出版信息

Ophthalmic Res. 1993;25(5):289-94. doi: 10.1159/000267327.

DOI:10.1159/000267327

PMID:8259261

Abstract

Nineteen cases of epiretinal membrane (ERM) in proliferative diabetic retinopathy were studied using immunohistochemical methods. Antibodies against type I-IV collagen, fibronectin (FN), glial fibrillary acidic protein (GFAP), vimentin and the monoclonal antibody (MAB) against human Müller cells were used to examine the membranes. Type II collagen was found on one side of the ERMs in 95% of the cases. The other types of collagen, FN and vimentin were also identified in most cases. Müller cells (GFAP- and MAB-positive) were found in 2/19 cases (11%), and astrocytes (GFAP-positive but MAB-negative) were found in 10/19 cases (53%). These results suggest that type II collagen (vitreal collagen) may act as a scaffolding in the formation of ERMs and that glial elements of the ERMs consist mainly of astrocytes.

摘要

采用免疫组织化学方法对19例增殖性糖尿病视网膜病变的视网膜前膜（ERM）进行了研究。使用抗I - IV型胶原、纤连蛋白（FN）、胶质纤维酸性蛋白（GFAP）、波形蛋白的抗体以及抗人Müller细胞的单克隆抗体（MAB）对这些膜进行检测。在95%的病例中，II型胶原存在于ERM的一侧。在大多数病例中也鉴定出了其他类型的胶原、FN和波形蛋白。在19例中有2例（11%）发现了Müller细胞（GFAP和MAB阳性），19例中有10例（53%）发现了星形胶质细胞（GFAP阳性但MAB阴性）。这些结果表明，II型胶原（玻璃体胶原）可能在ERM的形成中起到支架作用，并且ERM的胶质成分主要由星形胶质细胞组成。