Okada M, Matsumura M, Ogino N, Honda Y
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Graefes Arch Clin Exp Ophthalmol. 1990;228(5):467-74. doi: 10.1007/BF00927264.
We investigated the expression of vimentin and glial fibrillary acidic protein (GFAP) within Müller cells in normal human retinas and in detached human retinas of proliferative vitreoretinopathy (PVR) cases using the immunogold method. Müller cells in normal retinas showed vimentin immunoreactivity and faint GFAP immunoreactivity; however, in detached retinas they showed distinct GFAP immunoreactivity as well as vimentin immunoreactivity. Immunoelectron microscopic observation revealed that intermediate filaments (IF) within Müller cells in normal retinas showed vimentin immunoreactivity and that those within Müller cells in detached retinas showed both vimentin and GFAP immunoreactivity. Double staining for vimentin and GFAP showed that in detached retinas, these two protein immunoreactivities were observed in the same filaments. These results indicate that IF of human Müller cells consist of vimentin under normal conditions and that Müller cells in detached retinas contain different IF, which consist of vimentin and GFAP.
我们采用免疫金法，研究了正常人视网膜以及增殖性玻璃体视网膜病变（PVR）患者脱离的视网膜中，穆勒细胞内波形蛋白和胶质纤维酸性蛋白（GFAP）的表达情况。正常视网膜中的穆勒细胞显示波形蛋白免疫反应性以及微弱的GFAP免疫反应性；然而，在脱离的视网膜中，它们显示出明显的GFAP免疫反应性以及波形蛋白免疫反应性。免疫电镜观察显示，正常视网膜中穆勒细胞内的中间丝（IF）显示波形蛋白免疫反应性，而脱离视网膜中穆勒细胞内的中间丝则显示波形蛋白和GFAP免疫反应性。波形蛋白和GFAP的双重染色显示，在脱离的视网膜中，这两种蛋白免疫反应性出现在同一丝状物中。这些结果表明，正常情况下人类穆勒细胞的IF由波形蛋白组成，而脱离视网膜中的穆勒细胞含有不同的IF，其由波形蛋白和GFAP组成。
