Dunster L M, Schneider-Schaulies J, Löffler S, Lankes W, Schwartz-Albiez R, Lottspeich F, ter Meulen V
Institut für Virologie und Immunbiologie, Würzburg, Germany.
Virology. 1994 Jan;198(1):265-74. doi: 10.1006/viro.1994.1029.
Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.
麻疹病毒是一种极具传染性的病毒，可导致人类急性和持续性疾病，其受体仍未得到充分表征。我们分离出了一种单克隆抗体（mAb），命名为mAb 119，它能以剂量依赖的方式特异性抑制易感细胞系的麻疹病毒感染。该抗体从125I表面标记的细胞中沉淀出一种表观分子量为75 kDa的蛋白质，其表位存在于人类外周血单核细胞、人类细胞系以及非洲绿猴细胞系Vero中。用共价结合mAb 119的琼脂糖柱对去污剂溶解的细胞膜蛋白进行亲和层析，可对75 kDa的蛋白质进行部分纯化。将麻疹病毒与此亲和纯化的蛋白质预孵育，可剂量依赖性地抑制麻疹病毒感染。对该蛋白质进行氨基酸微量测序，结果显示它与人类膜组织延伸刺突蛋白埃兹蛋白相同，埃兹蛋白是一种在细胞内外与细胞质膜相关的蛋白质。随后，发现针对纯化埃兹蛋白产生的抗体（mAb 38/87）也能特异性抑制易感细胞的麻疹病毒感染，并证实了我们用mAb 119获得的数据。我们的数据表明，埃兹蛋白充当麻疹病毒的受体。
