Ciccaglione A R, Rapicetta M, Fabiano A, Argentini C, Silvestro M, Giuseppetti R, Varano F, D'Urso N, Dinolfo L, Morgando A
Department of Virology, Istituto Superiore di Sanità, Rome, Italy.
Arch Virol Suppl. 1993;8:15-21. doi: 10.1007/978-3-7091-9312-9_2.
Two woodchucks (Marmota monax) intrahepatically inoculated with hepatitis delta virus (HDV) complementary DNA clones pSVL-D3 and pSVL-Ag showed virological and pathological signs of acute and chronic HDV infection. HDV-RNA and hepatitis delta antigen (HDAg) were detected in serum by slot-blot hybridization and by western blot five weeks after inoculation. Liver biopsy specimens collected at 8th week post inoculum were positive for HDV-RNA. Anti-HDV antibodies were detected at the 11th and 9th weeks, respectively. Histological finding of hepatocarcinoma and persistence of circulating HDV-RNA and anti-HDV were observed up to the 10th month. Both woodchucks produced "small" and "large" HDAg antigen, although the inoculated cloned DNA bears the coding capability solely for the small antigen. A transient decrease of woodchuck hepatitis virus DNA (WHV-DNA) level was observed during the peak of HDV infection. Successive inoculation of acute-phase serum in three woodchucks resulted in a successful infection in one of the animals.
两只经肝内接种丁型肝炎病毒（HDV）互补DNA克隆pSVL - D3和pSVL - Ag的土拨鼠（Marmota monax）表现出急性和慢性HDV感染的病毒学和病理学迹象。接种五周后，通过斑点杂交和蛋白质印迹法在血清中检测到HDV - RNA和丁型肝炎抗原（HDAg）。接种后第8周采集的肝活检标本HDV - RNA呈阳性。分别在第11周和第9周检测到抗HDV抗体。直至第10个月，观察到肝癌的组织学表现以及循环HDV - RNA和抗HDV的持续存在。两只土拨鼠均产生了“小”和“大”HDAg抗原，尽管接种的克隆DNA仅具有编码小抗原的能力。在HDV感染高峰期观察到土拨鼠肝炎病毒DNA（WHV - DNA）水平短暂下降。在三只土拨鼠中连续接种急性期血清，其中一只动物成功感染。
