Norder H, Couroucé A M, Magnius L O
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Arch Virol Suppl. 1993;8:189-99. doi: 10.1007/978-3-7091-9312-9_19.
The complete nucleotide sequences of six hepatitis B viral (HBV) genomes were determined by dideoxy chain termination sequencing of ten overlapping nucleotide fragments obtained by the polymerase chain reaction. Four of the genomes belonged to the two genomic groups E and F of HBV which have been previously identified by us on the basis of sequence divergences within the S gene. Genomic group E encodes the HBsAg subtype ayw4, group F adw4q-. The other two genomes were of Pacific origin within group C and encoded adrq-. The relationship of these complete human HBV genomes to 21 that have been previously published, together with one chimpanzee virus and four rodent hepadnaviral genomes, was investigated by constructing a phylogenetic tree utilizing a combination of distance matrix and approximate parsimonious methods. Thereby, the previously demonstrated segregation of human HBV strains into six genomic groups was confirmed. Both of the representatives of the groups E and F were found to differ by 8.1-13.6% and by 12.8-15.5% from the genomes of the other genomic groups and by 1.5 and 3.7% from each other. Since they differed by more than 8% from the genomes in the other groups, the limit originally used to define HBV, genomic groups their status as new genomic groups was confirmed. The two Pacific group C strains were found to differ by 2.7% from each other and by 4.1 to 5.4% from other group C genomes, suggesting that they diverged early from the other group C genomes. According to both the overall similarity and the phylogenetic dendrogram the F strains formed the most divergent cluster of HBV genomes favoring the concept that they represented the original HBV strains of the New World. The next split in the dendrogram segregated the A, D, E and the chimpanzee strains from the Asian B and C strains. Information on the nucleotide sequences and their encoded products of HBV strains of different genomic groups will provide a basis to understand biological variations of the HBV infection in different parts of the world.
通过对聚合酶链反应获得的十个重叠核苷酸片段进行双脱氧链终止测序，确定了六个乙型肝炎病毒（HBV）基因组的完整核苷酸序列。其中四个基因组属于我们先前根据S基因内的序列差异鉴定出的HBV的两个基因组群E和F。基因组群E编码HBsAg亚型ayw4，基因组群F编码adw4q-。另外两个基因组来自C组的太平洋地区，编码adrq-。通过使用距离矩阵和近似简约方法相结合构建系统发育树，研究了这些完整的人类HBV基因组与之前发表的21个基因组、一个黑猩猩病毒基因组和四个啮齿动物嗜肝DNA病毒基因组之间的关系。由此，证实了先前证明的人类HBV毒株分为六个基因组群的情况。发现基因组群E和F的两个代表与其他基因组群的基因组相比，差异分别为8.1%-13.6%和12.8%-15.5%，彼此之间差异为1.5%和3.7%。由于它们与其他组的基因组差异超过8%，最初用于定义HBV基因组群的界限得到了确认，它们作为新基因组群的地位也得到了确认。发现两个太平洋C组毒株彼此之间差异为2.7%，与其他C组基因组差异为4.1%-5.4%，这表明它们在早期就与其他C组基因组发生了分化。根据总体相似性和系统发育树状图，F组毒株形成了HBV基因组中最具分歧的簇，这支持了它们代表新世界原始HBV毒株的概念。系统发育树状图中的下一个分支将A、D、E组毒株和黑猩猩毒株与亚洲B组和C组毒株分开。不同基因组群的HBV毒株的核苷酸序列及其编码产物的信息将为理解世界不同地区HBV感染的生物学变异提供基础。
