Grishin E V, Himmelreich N H, Pluzhnikov K A, Pozdnyakova N G, Storchak L G, Volkova T M, Woll P G
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
FEBS Lett. 1993 Dec 27;336(2):205-7. doi: 10.1016/0014-5793(93)80803-3.
We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.
我们研究了由两种多肽（130 kDa的α-拉毒素（α-LTX）和8 kDa的低分子量蛋白（LMWP））组成的α-拉毒素复合物的作用，以及含有过量LMWP的含α-LTX毒液组分对突触体和PC12细胞钙内流以及突触体[14C]γ-氨基丁酸（GABA）释放的作用。两种制剂均能显著激活钙内流并刺激突触体释放[14C]GABA。将两种制剂与针对LMWP 14个氨基酸残基C末端肽的抗体预孵育，可对这些效应产生不同的调节作用。抗体可抑制诱导的钙内流并增强诱导的GABA释放。
