Volkova T M, Pluzhnikov K A, Woll P G, Grishin E V
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Toxicon. 1995 Apr;33(4):483-9. doi: 10.1016/0041-0101(94)00166-6.
Highly purified alpha-latrotoxin from the black widow spider venom (alpha-LTX) consists of two polypeptides with mol. wts of 130,000 and 8000 (LMWP). We have isolated two low mol. wt proteins LMWP and LMWP2 from the low mol. wt fraction of this venom. The chemical properties of these proteins and partial amino acid sequence of novel protein LMWP2 were studied. By means of i.v. or intracerebroventricular injections into mice it was shown that low mol. wt components of the venom at concentrations of 2.3 mg/kg and 0.8 mg/kg, respectively, did not possess any direct toxic effect on vertebrates. Injections of each protein into the third thoracic segment of cockroaches Periplaneta americana (doses up to 80 micrograms/g) did not cause lethality or paralysis of insects.
从黑寡妇蜘蛛毒液中高度纯化的α-拉托毒素（α-LTX）由两种分子量分别为130,000和8000的多肽组成（低分子量多肽，LMWP）。我们从这种毒液的低分子量组分中分离出了两种低分子量蛋白质LMWP和LMWP2。研究了这些蛋白质的化学性质以及新型蛋白质LMWP2的部分氨基酸序列。通过静脉注射或脑室内注射到小鼠体内的实验表明，毒液的低分子量组分在浓度分别为2.3mg/kg和0.8mg/kg时，对脊椎动物没有任何直接毒性作用。将每种蛋白质注射到美洲大蠊的第三胸节（剂量高达80微克/克）中，不会导致昆虫死亡或麻痹。
