Yáñez R J, Rodríguez J M, Boursnell M, Rodríguez J F, Viñuela E
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Gene. 1993 Dec 8;134(2):161-74. doi: 10.1016/0378-1119(93)90090-p.
Two open reading frames (ORFs) of African swine fever virus (ASFV) encoding putative helicases have been sequenced. The two genes, termed D1133L and B962L, are located in the central region of the viral genome, but are separated by about 40 kb of DNA. Both genes are expressed late during ASFV infection of Vero cells, after replication of viral DNA has begun. Contiguous to D1133L, three other ORFs (D129L, D79L and D339L), encoding putative proteins of unknown function, have been sequenced. Proteins D1133L and B962L contain the amino acid motifs that characterize helicases of superfamily II. D1133L is most similar to a group of putative helicases which includes two proteins of vaccinia virus (D11L and D6R) involved in transcription of the viral genome, their homologues in other poxviruses, the protein encoded by ORF 4 of the yeast plasmids, pGKL2 and pSKL, and the previously identified ASFV protein, Q706L. B962L resembles a group of RNA-helicase-like proteins which includes three proteins of Saccharomyces cerevisiae involved in pre-mRNA splicing (PRP2, PRP16 and PRP22), Drosophila melanogaster KURZ and MLE, and vaccinia virus 18R.
已对非洲猪瘟病毒（ASFV）编码假定解旋酶的两个开放阅读框（ORF）进行了测序。这两个基因，分别称为D1133L和B962L，位于病毒基因组的中央区域，但被约40 kb的DNA隔开。在Vero细胞感染ASFV期间，这两个基因均在病毒DNA开始复制之后的晚期表达。与D1133L相邻，已对另外三个开放阅读框（D129L、D79L和D339L）进行了测序，它们编码功能未知的假定蛋白质。蛋白质D1133L和B962L含有表征超家族II解旋酶的氨基酸基序。D1133L与一组假定解旋酶最为相似，这组解旋酶包括痘苗病毒的两种蛋白质（D11L和D6R），它们参与病毒基因组的转录，其他痘病毒中的同源物，酵母质粒pGKL2和pSKL的ORF 4编码的蛋白质，以及先前鉴定的ASFV蛋白质Q706L。B962L类似于一组RNA解旋酶样蛋白质，其中包括酿酒酵母中参与前体mRNA剪接的三种蛋白质（PRP2、PRP16和PRP22）、黑腹果蝇的KURZ和MLE，以及痘苗病毒18R。
