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High-performance liquid chromatographic determination of histatins in human saliva.

作者信息

Sugiyama K, Ogata K

机构信息

Department of Pharmacology, Okayama University Dental School, Japan.

出版信息

J Chromatogr. 1993 Sep 22;619(2):306-9. doi: 10.1016/0378-4347(93)80121-j.

DOI:10.1016/0378-4347(93)80121-j
PMID:8263103
Abstract

A simple method for the assay of histatins in human saliva by high-performance liquid chromatography has been developed. The histatins in saliva were extracted with 0.1 M HCl-methanol, and histatins 1, 3, 5, and 6 were separated and concentrations were determined by reversed-phase chromatography. This simple method enabled the determination of levels of individual histatins from the saliva of normal subjects. The average concentration of histatins 1, 3, 5, and 6 in parotid saliva collected from 26 healthy volunteers aged from 20 to 30 years were 11.25 +/- 5.65, 8.15 +/- 3.08, 7.67 +/- 3.12, and 1.56 +/- 0.53 mumol/l (mean +/- S.D.), respectively.

摘要

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