Altonen M, SAXEN L, Kosunen T, Ainamo J
Int J Oral Surg. 1976 Dec;5(6):276-84. doi: 10.1016/s0300-9785(76)80028-2.
Two degerming mouthrinses, a 1% povidone-iodine and a 0.2% aqueous solution of chlorhexidine gluconate, were tested with regard to their degerming effect on the saliva. The study comprised two parts. In the first part 19 dental students with good oral hygiene rinsed their mouths at weekly intervals with 10 ml of the povidone-iodine and 10 ml of the chlorhexidine solution. A control group of 12 students and nurses with healthy teeth and gums rinsed their mouths with 10 ml of plain water. In the second part 11 adult patients with periodontal disease used the two test rinses at weekly intervals both before and after periodontal prophylaxis including scaling of the teeth. Non-stimulated saliva was simpled immediately before and 5, 30, 60, and 120 min after each rinse. Part of the saliva was cultured on blood agar plates to show the growth of aerobic microbes. The remainder of the saliva was poured over a Dentocult dip-slide for determination of the number of acidophilic bacteria. The results showed that in the water control group the bacterial count increased in spite of the rinse. When compared with the prerinse values, both test rinses clearly reduced the amounts of bacteria. Chlorhexidine reduced the bacterial count 5 min after the rinse about one logarithm more than povidone-iodine, did, and the degerming effect of chlorhexidine was also of longer duration than that of the povidone-iodine solution. The periodontal prophylaxis in the adult group did not seem to lower the pre-rinse bacterial counts but did slightly improve the duration of the effect of both test solutions. The dip-slide tests showed that 28% of the subjects had no growth of acidophilic bacteria. This absence was not dependent on the other bacterial flora of the saliva. In the cases with a positive dip-slide test, the number of acidophilic bacteria decreased with both test solutions according to the pattern revealed by the blood agar plate cultures.
对两种消毒漱口水，即1%聚维酮碘和0.2%葡萄糖酸氯己定水溶液，就其对唾液的消毒效果进行了测试。该研究包括两个部分。在第一部分中，19名口腔卫生良好的牙科学生每周用10毫升聚维酮碘和10毫升氯己定溶液漱口。一个由12名牙齿和牙龈健康的学生和护士组成的对照组用10毫升清水漱口。在第二部分中，11名患有牙周病的成年患者在包括洗牙在内的牙周病预防前后每周使用这两种测试漱口水。在每次漱口前以及漱口后5、30、60和120分钟立即采集非刺激性唾液。部分唾液在血琼脂平板上培养以显示需氧微生物的生长。其余唾液倾注在Dentocult浸片上以测定嗜酸菌的数量。结果表明，在清水对照组中，尽管进行了漱口，细菌数量仍有所增加。与漱口前的值相比，两种测试漱口水都明显减少了细菌数量。氯己定在漱口后5分钟使细菌数量减少的幅度比聚维酮碘多约一个对数级，并且氯己定的消毒效果持续时间也比聚维酮碘溶液长。成年组的牙周病预防似乎并未降低漱口前的细菌数量，但确实略微延长了两种测试溶液的效果持续时间。浸片测试表明，28%的受试者没有嗜酸菌生长。这种情况与唾液中的其他细菌菌群无关。在浸片测试呈阳性的病例中，两种测试溶液都使嗜酸菌数量按照血琼脂平板培养显示的模式减少。
