Dineen P
Clin Pharmacol Ther. 1978 Jan;23(1):63-7. doi: 10.1002/cpt197823163.
Two antiseptic preparations for hand washing were compared by the glove-juice method in a crossover study on 10 volunteers. The reference preparation was 7.5% povidone-iodine (Betadine Surgical Scrub); the test agent was 4% chlorhexidine gluconate combined with 4% isopropyl alcohol (Hibiclens). The experimental model included inoculation of the hands with a mixture of Serratia marcescens, Escherichia coli, Providentia stuartii, and Pseudomonas aeruginosa. The reference preparation achieved a reduction ratio in colony counts of 695 to 1 under the conditions of this study. The average postwash colony count after use of 7.5% povidone-iodine was significantly less than the preinoculation colony count. Logarithmic values, and the paired t test applied to them, showed a highly significant difference (p = less than 0.001) in favor of the degerming ability of the reference agent compared to the test agent. These data are of value in the selection of preparations for hand washing and may point the way to quantitative methods for other degerming studies.
在一项针对10名志愿者的交叉研究中，采用手套浸液法比较了两种用于洗手的抗菌制剂。参比制剂为7.5%聚维酮碘（碘伏外科 scrub）；受试制剂为4%葡萄糖酸氯己定与4%异丙醇的复方制剂（洗必泰醇）。实验模型包括用粘质沙雷氏菌、大肠埃希菌、斯氏普罗威登斯菌和铜绿假单胞菌的混合物对手部进行接种。在本研究条件下，参比制剂的菌落计数减少率达到695:1。使用7.5%聚维酮碘后洗手后的平均菌落计数显著低于接种前的菌落计数。对数数值以及应用于它们的配对t检验显示，与受试制剂相比，参比制剂的除菌能力存在高度显著差异（p＜0.001）。这些数据对于洗手制剂的选择具有价值，并且可能为其他除菌研究的定量方法指明方向。
