Borgeat P, Hamberg M, Samuelsson B
J Biol Chem. 1976 Dec 25;251(24):7816-20.
Addition of arachidonic acid and homo-gamma-linolenic acid to a suspension of rabbit peritoneal neutrophils led to the synthesis of 5-L-hydroxy-6,8,11,14-eicosatetraenoic acid and 8-L-hydroxy-9,11,14-eicosatrienoic acid, respectively. Both hydroxy acids were found to be the main metabolites of their respective unsaturated C-20 fatty acid precursor, constituting more than 50% of the total substrate conversion. The formation of the two metabolites was not inhibted bb indomethacin, indicating that the enzymes involved were unrelated to the prostaglandin synthetase system. The presence in the two compounds of a hydroxyl group alpha to a pair of conjugated cis/trans double bonds suggested that they were formed by action of lipoxygenease(s).
将花生四烯酸和高γ-亚麻酸添加到兔腹膜中性粒细胞悬液中，分别导致5-L-羟基-6,8,11,14-二十碳四烯酸和8-L-羟基-9,11,14-二十碳三烯酸的合成。发现这两种羟基酸分别是其各自不饱和C-20脂肪酸前体的主要代谢产物，占总底物转化的50%以上。这两种代谢产物的形成不受吲哚美辛的抑制，表明所涉及的酶与前列腺素合成酶系统无关。这两种化合物中在一对共轭顺式/反式双键的α位存在一个羟基，表明它们是由脂氧合酶作用形成的。
