O'Shea T, Bindon B M, Forage R G, Findlay J K, Tsonis C G
Department of Physiology, University of New England, Armidale, NSW, Australia.
Reprod Fertil Dev. 1993;5(2):173-80. doi: 10.1071/rd9930173.
Ewe lambs (n = 24-25) were immunized at 3, 7 and 15 weeks of age with recombinant bovine alpha-inhibin (rec inhibin) or with bovine monoclonal antibody purified inhibin (bMPI) obtained by immunochromatography from bovine follicular fluid or with adjuvant alone (control). Antibodies in the plasma of the lambs immunized with the inhibin preparations bound to iodinated 31 kDa bovine inhibin. Binding was minimal after the primary immunization, increased after each booster immunization and remained elevated until at least 45 weeks of age (29% for rec inhibin and 11% for bMPI). Of the group treated with rec inhibin, 10 ovulated as lambs (control 0/22; bMPI 3/21) and onset of overt oestrous activity (as hoggets) was advanced (P < 0.05) by 17 days in lambs immunized with rec inhibin. As hoggets, the ovulation rate was greater (P < 0.01) in the rec inhibin immunized lambs (4.41 +/- 0.67) than in the control animals (1.27 +/- 0.15) but not in the bMPI-treated lambs (1.40 +/- 0.16). After a further immunization at 17 months of age, however, ovulation rate increased (P < 0.01) in the bMPI-immunized group (3.40 +/- 0.47) but was unchanged in the lambs immunized with rec inhibin (2.80 +/- 0.52) and those in the control group (1.15 +/- 0.08). There were no effects of immunization on plasma concentrations of either follicle stimulating hormone (FSH) or luteinizing hormone (LH). The initial smaller response seen with the bMPI may be due to either the presence of compounds other than inhibin in such preparations or the small absolute amount of inhibin injected.(ABSTRACT TRUNCATED AT 250 WORDS)
对24至25只母羊羔羊在3周、7周和15周龄时分别用重组牛α -抑制素（重组抑制素）、通过免疫层析从牛卵泡液中获得的牛单克隆抗体纯化抑制素（bMPI）或仅用佐剂（对照）进行免疫。用抑制素制剂免疫的羔羊血浆中的抗体与碘化的31 kDa牛抑制素结合。初次免疫后结合最少，每次加强免疫后增加，并且至少在45周龄前一直保持升高（重组抑制素组为29%，bMPI组为11%）。在接受重组抑制素治疗的组中，10只羔羊作为幼羊排卵（对照组22只中0只；bMPI组21只中3只），并且用重组抑制素免疫的羔羊作为育成羊时明显发情活动的开始提前了17天（P < 0.05）。作为育成羊，用重组抑制素免疫的羔羊排卵率（4.41±0.67）高于对照动物（1.27±0.15）（P < 0.01），但bMPI处理的羔羊（1.40±0.16）则不然。然而，在17月龄进一步免疫后，bMPI免疫组的排卵率增加（P < 0.01）（3.40±0.47），而用重组抑制素免疫的羔羊（2.80±0.52）和对照组羔羊（1.15±0.08）的排卵率没有变化。免疫对促卵泡激素（FSH）或促黄体生成素（LH）的血浆浓度没有影响。bMPI最初较小的反应可能是由于此类制剂中除抑制素外还存在其他化合物，或者是注射的抑制素绝对量较小。（摘要截断于250字）
